murine fibroblast l929 cells  (ATCC)

Journal: bioRxiv

Article Title: Structure Elucidation, Biosynthesis and Biological Evaluation of Neosorangicin A, a Member of the Sorangicin Family

doi: 10.64898/2026.01.26.701680

Figure Lengend Snippet: Intracellular activity of sorangicins against S. aureus Newman in A549 ( A ) and NIH 3T3 ( B ) cells. At 1.75 hours post infection, cells were washed once and treated with different concentrations of rifampicin (RIF), sorangicin A (SorA), neosorangicin A (NeoSorA), or with 0.1% ( v/v ) methanol (control) in the presence of 10 µg mL −1 gentamicin. Reduction of bacterial burden was evaluated 24 hours post infection by CFU (colony-forming unit) counting. Limit of detection (LoD) is 10 1 CFU. Horizontal lines represent mean values of at least three independent biological experiments. Statistical analysis was performed by one-way ANOVA with Dunnett’s multiple comparison test. p < 0.05: *, p < 0.0001: ****.

Article Snippet: Human alveolar epithelial A549 cells (ATCC CCL-185) and murine fibroblast NIH 3T3 cells (ATCC CRL-1658), obtained from the American Type Culture Collection, were cultured in DMEM with 4.5 g/L glucose, 10% fetal bovine serum, 2 mM L -glutamine, 1mM sodium pyruvate and 1% non-essential amino acids at 37 °C and 7.5% CO 2 .

Techniques: Activity Assay, Infection, Control, Comparison